Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)
Identifieur interne : 000849 ( Main/Exploration ); précédent : 000848; suivant : 000850Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)
Auteurs : Ester Santigosa [Espagne] ; Miguel Ngel Sáenz De Rodrigá Ez [Espagne] ; Ana Rodiles [Espagne] ; Fernando García Barroso [Espagne] ; Francisco Javier Alarc N [Espagne]Source :
- Aquaculture Research [ 1355-557X ] ; 2010-08.
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Abstract
Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g kg−1 of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor‐supplemented diets when compared with those fed on an inhibitor‐free diet. Only the SGR at day 15 decreased significantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in fish fed on inhibitor‐supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed on the highest level of soybean inhibitor (4.0 g kg−1) was also observed. However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor‐free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all fish were maintained under experimentation for 10 more days and fed on an inhibitor‐free diet to determine whether the possible effects caused by the protease inhibitor could be reverted. The administration of SBTI‐supplemented diets did not affect sea bream growth performance or intestine histology after 30 days, and only a decrease in the total alkaline protease activity was found at day 15.
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DOI: 10.1111/j.1365-2109.2010.02500.x
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The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor‐supplemented diets when compared with those fed on an inhibitor‐free diet. Only the SGR at day 15 decreased significantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in fish fed on inhibitor‐supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed on the highest level of soybean inhibitor (4.0 g kg−1) was also observed. However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor‐free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all fish were maintained under experimentation for 10 more days and fed on an inhibitor‐free diet to determine whether the possible effects caused by the protease inhibitor could be reverted. The administration of SBTI‐supplemented diets did not affect sea bream growth performance or intestine histology after 30 days, and only a decrease in the total alkaline protease activity was found at day 15.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Catalogne</li></region><settlement><li>Barcelone</li></settlement></list><tree><country name="Espagne"><region name="Catalogne"><name sortKey="Santigosa, Ester" sort="Santigosa, Ester" uniqKey="Santigosa E" first="Ester" last="Santigosa">Ester Santigosa</name></region><name sortKey="Alarc N, Francisco Javier" sort="Alarc N, Francisco Javier" uniqKey="Alarc N F" first="Francisco Javier" last="Alarc N">Francisco Javier Alarc N</name><name sortKey="Barroso, Fernando Garcia" sort="Barroso, Fernando Garcia" uniqKey="Barroso F" first="Fernando García" last="Barroso">Fernando García Barroso</name><name sortKey="Rodiles, Ana" sort="Rodiles, Ana" uniqKey="Rodiles A" first="Ana" last="Rodiles">Ana Rodiles</name><name sortKey="Saenz De Rodriga Ez, Miguel Ngel" sort="Saenz De Rodriga Ez, Miguel Ngel" uniqKey="Saenz De Rodriga Ez M" first="Miguel Ngel" last="Sáenz De Rodrigá Ez">Miguel Ngel Sáenz De Rodrigá Ez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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